Coding

Part:BBa_K942007:Design

Designed by: Luis Mario Leal Garza   Group: iGEM12_Tec-Monterrey   (2012-09-26)

SS+Api m 6+His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Signal Sequence at the N-terminal of this protein is useful for extracellular secretion. Its purification can be done by affinity to metallic ions with 6x His tag at the C-Terminal. Api m 6 codon optimization is done to be better expressed in yeasts.

Source

The Api m 6 allergen native from Apis miellifera, is an allergen from the bee venom.

References