Coding
Part:BBa_K942007:Design
Designed by: Luis Mario Leal Garza Group: iGEM12_Tec-Monterrey (2012-09-26)
SS+Api m 6+His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Signal Sequence at the N-terminal of this protein is useful for extracellular secretion. Its purification can be done by affinity to metallic ions with 6x His tag at the C-Terminal. Api m 6 codon optimization is done to be better expressed in yeasts.
Source
The Api m 6 allergen native from Apis miellifera, is an allergen from the bee venom.